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Analysis of NT-proANP (Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0464-LGZ)

Introduction

Cardiovascular markers refer to markers that can be sideloged in circulating blood and can sensitively and specifically reflect myocardial injury (abnormal) and its severity. Cardiovascular markers, which normally exist mainly or only in the heart (myocardium) and are released by the heart in large quantities in the case of cardiac or cardiovascular abnormalities, can be used for screening, diagnosis, prognosis assessment and follow-up treatment of a series of circulatory diseases involving the heart, blood vessels (arteries, veins, and microvessels). The markers of cardiac function include type A natriuretic peptide (ANP), type B natriuretic peptide (BNP) and its corresponding non-bioactive complex component (NT-proANP, NT-proBNP).




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiac Biomarkers, Cardiac Injury, Natriuretic Peptide, Toxicology, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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