Analysis of Osteonectin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0470-LGZ)

Introduction

Osteonectin (ON) is an acid phosphorylated glycoprotein with a molecular weight of 43 kDa. This protein is a single-stranded polypeptide consisting of a signal sequence of 17 amino acids and 283 to 287 amino acids (depending on the mammalian species), and contains four functional domains (I to IV).

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Bone Disorders

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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