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Analysis of Eotaxin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0147-LGZ)

Introduction

Eotaxin is a potent chemoattractant for eosinophils. It is a member of the CC chemokine family and it is expressed only in the adult gut, thymus and skin. The human factor consists of 74 amino acid residues. Its members are produced by lung and bronchial epithelial cells, induced by interleukin-4, and repressed by interferon gamma. Mainly responsible for the infiltration of eosinophils into the site of allergic inflammation.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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