Analysis of PDGFRB Gene Rearrangement by Southern Blot Technology (CAT#: STEM-MHT-0016-LGZ)

Introduction

Official Full Name: platelet derived growth factor receptor beta
Also known as: IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for mesenchymal cells. The identity of the growth factor bound to the receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for the normal development of the cardiovascular system and contributes to the rearrangement of the actin cytoskeleton. The gene is flanked on chromosome 5 by the granulocyte-macrophage colony-stimulating factor and macrophage colony-stimulating factor receptor genes; all three genes may be associated with 5-q syndrome. A translocation between chromosomes 5 and 12, which fuses this gene to the ETV6 gene, causes a chronic myeloproliferative disorder with eosinophilia.

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Principle Applications Procedure Materials Notes Related Products

Principle

Under certain conditions, two single strands of nucleic acid with certain homology can be specifically hybridized to form double strands according to the principle of base complementarity. Generally, DNA molecules to be detected are digested with restriction enzymes, separated by agar-gel electrophoresis, denatured and transferred to nitrocellulocellulose film or nylon film or other solid phase support according to their position in the gel, fixed and then reacted with DNA probes labeled with isotopes or other markers. This is followed by autoradiography or an enzyme reaction to detect the amount of specific DNA molecules. If the object to be tested contains a sequence that is complementary to the probe, the two are combined by the principle of base complementarity, and the free probe is washed and detected by self-development or other suitable techniques, thus revealing the fragment to be tested and its relative size.

Applications

Gene Rearrangement Detection

Procedure

1. Sample Processing
2. DNA Extraction and Digestion
3. Gel Electrophoresis
4. Gel Pretreatment
5. Transfer membrane
6. Probe Labeling
7. Prehybridization (blocking)
8. Southern hybridization
9. Membrane washing
10. Autoradiographic Assay
11. Results Analysis

Materials

Sample: DNA, Bacterial Fluid/Tissue/Cell

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