Analysis of PPARG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1833-LGZ)

Introduction

Official Full Name: peroxisome proliferator activated receptor gamma
Also known as: GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs), and these heterodimers regulate the transcription of various genes. Three subtypes of ppar are known: ppar-alpha, ppar-delta and ppar-gamma. The protein encoded by this gene is ppar-gamma, a regulator of adipocyte differentiation. In addition, PPAR-γ has been implicated in the pathology of many diseases, including obesity, diabetes, atherosclerosis, and cancer. Alternately spliced transcript variants encoding different isoforms have been described.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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