Analysis of Pyruvic Acid by Enzyme-labeled Instrument (CAT#: STEM-MB-1404-LGZ)

Introduction

Sensitivity: 0.003 μmol/mL
Detection range: 0.003-2.0 μmol/mL
Precision: inter-lot difference of 3.6%, intra-lot difference of 2.3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 480-520 nm

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Principle Applications Procedure Materials

Principle

Pyruvic acid and color reagent reaction, reaction product in the alkaline solution is reddish brown, the color is proportional to the content of pyruvic acid, through the colorimetric method can calculate the content of pyruvic acid.

Applications

Suitable for detection of pyruvic acid content in serum (plasma), animal and plant tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, animal and plant tissues

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