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Analysis of SAG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1783-LGZ)

Introduction

Official Full Name: S-antigen visual arrestin<br />Also known as: RP47; RP96; S-AG<br />Members of the arrestin/β-arrestin family are thought to be involved in agonist-mediated desensitization of G protein-coupled receptors and cause specific inhibition of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals . S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein involved in desensitization of light-activated transduction cascades. It is expressed in the retina and pineal gland and inhibits the conjugation of rhodopsin to transducin in vitro. Furthermore, s-arrestin is highly antigenic and capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene are associated with Oguchi disease, a rare form of autosomal recessive night blindness.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements