Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of sCD163 by ELISA (CAT#: STEM-MB-1593-LGZ)

Introduction

CD163 is a protein encoded in humans by the CD163 gene. It belongs to the cysteine-rich B-type scavinger receptor family, which contains an extracellular domain of 1048 amino acid residues, a single transmembrane segment, and a cytoplasmic tail region with several splicing variants. It is a scavenger receptor of the hemoglobin-bound globin complex and can label monocyte/macrophage lineages. The soluble form of this receptor is found in plasma and is usually represented as sCD163. sCD163 is produced by extracellular shedding of membrane bound receptors and is upregulated in a wide range of inflammatory diseases including cirrhosis, type 2 diabetes, macrophage activation syndrome, Gorscher's disease, sepsis, HIV infection, rheumatoid arthritis, and Hodgkin's lymphoma.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
Advertisement