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Analysis of SCF (Human) by ELISA (CAT#: STEM-MB-1172-LGZ)

Introduction

Stem cell factor (SCF), also known as c-kit ligand, is a basic hematopoietic stem cell growth factor with anti-apoptotic function. The human SCF gene encodes a leader peptide, an extracellular portion of 189 amino acids, a hydrophobic transmembrane region and an intracellular region. Natural SCF exists in a membrane-bound form or in a soluble form. The soluble portion is 164 or 165 amino acids from the beginning of the extracellular portion.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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