Analysis of SELL Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0650-LGZ)

Introduction

Official Full Name: selectin L
Also known as: TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
This gene encodes a cell surface adhesion molecule and belongs to the adhesion/homing receptor family. The encoded protein contains a c-type lectin-like domain, a calcium-binding epidermal growth factor-like domain and two short complement-like repeats. This gene product is required for the binding and subsequent rolling of leukocytes on endothelial cells, facilitating their migration to secondary lymphoid organs and sites of inflammation. Single nucleotide polymorphisms in this gene have been associated with various diseases, including immunoglobulin A nephropathy. Alternative splicing transcript variants of this gene have been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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