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Analysis of SMG1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2469-LGZ)

Introduction

Official Full Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Also known as: ATX; LIP; 61E3.4
This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. This protein has kinase activity and is thought to function in NMD by phosphorylating a regulator of the nonsense transcript 1 protein. Another splice transcript variant has been described, but its full-length nature has not been determined.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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