Analysis of SMG1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2469-LGZ)

Introduction

Official Full Name: SMG1 nonsense mediated mRNA decay associated PI3K related kinase<br />Also known as: ATX; LIP; 61E3.4<br />This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. This protein has kinase activity and is thought to function in NMD by phosphorylating a regulator of the nonsense transcript 1 protein. Another splice transcript variant has been described, but its full-length nature has not been determined.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Haemonchus Similis by Real-Time PCR Method (CAT#: STEM-MB-3729-LGZ)
Analysis of CEP350 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0237-LGZ)
Analysis of IL10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1071-LGZ)
Analysis of Alcaligenes Faecalis by Real-Time PCR Method (CAT#: STEM-MB-3170-LGZ)
Analysis of EBV by Real-Time PCR Method (CAT#: STEM-MB-2554-LGZ)
Analysis of TMEM82 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1233-LGZ)
Analysis of Equine Rhinitis Virus by Real-Time PCR Method (CAT#: STEM-MB-2971-LGZ)
Analysis of FMO5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0998-LGZ)