Analysis of SMYD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0313-LGZ)

Introduction

Official Full Name: SET and MYND domain containing 3<br />Also known as: KMT3E; ZMYND1; ZNFN3A1; bA74P14.1<br />This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of DNM3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0916-LGZ)
Analysis of Mycobacterium Pinnipedii by Real-Time PCR Method (CAT#: STEM-MB-2959-LGZ)
Analysis of TIPRL Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0707-LGZ)
Analysis of TM2D2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1882-LGZ)
Analysis of OR51G1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2329-LGZ)
Analysis of ZNF281 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0274-LGZ)
Analysis of RPL5 Gene (Mutation) in Diamond-Blackfan anemia by RT-qPCR (CAT#: STEM-MT-0116-LGZ)
Analysis of LOC122889011 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1596-LGZ)