Inquiry Basket

Analysis of ST3GAL5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1692-LGZ)

Introduction

Official Full Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Also known as: SATI; SIAT9; SPDRS; ST3GalV; SIATGM3S; ST3Gal V
Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Yaba Monkey Tumor Virus (YMTV) by Real-Time PCR Method (CAT#: STEM-MB-3797-LGZ)
Analysis of FCER1G Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0961-LGZ)
Analysis of OR2B11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0531-LGZ)
Analysis of Klebsiella Pnenmoniae by Real-Time PCR Method (CAT#: STEM-MB-3455-LGZ)
Analysis of Columbid Herpes Virus 1 (CHV-1) by Real-Time PCR Method (CAT#: STEM-MB-3730-LGZ)
Analysis of Muscovy Duck Parvovirus (MDPV) by Real-Time PCR Method (CAT#: STEM-MB-3527-LGZ)
Analysis of Canine Parvo Virus(CPV) by Real-Time PCR Method (CAT#: STEM-MB-2688-LGZ)
Analysis of Cooperia Surnabada by Real-Time PCR Method (CAT#: STEM-MB-2622-LGZ)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies