Analysis of STK33 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2159-LGZ)

Introduction

Official Full Name: serine/threonine kinase 33
Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in mitotic DNA damage checkpoint signaling and protein autophosphorylation. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in cytoplasm and nucleus.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of MAP4K3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1732-LGZ)
Analysis of LOC126806396 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1489-LGZ)
Analysis of Human Immunodeficiency Virus 1 (HIV-1N) by Real-Time PCR Method (CAT#: STEM-MB-3212-LGZ)
Analysis of Corynebacterium Minutissimum by Real-Time PCR Method (CAT#: STEM-MB-2621-LGZ)
Analysis of RABGAP1L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0333-LGZ)
Analysis of VIT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1810-LGZ)
Analysis of CTRC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1298-LGZ)
Analysis of RIIAD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0616-LGZ)