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Analysis of TREM-1 (Rat, Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0604-LGZ)

Introduction

TREM1 (Triggering receptor expressed on the surface of myeloid cells-1) is a myeloid cell surface receptor expressed on the surface of neutrophils, monocytes, and macrophages, and is expressed in a variety of tumor microenvironments, synergizing with the classical pattern recognition receptor (PRR). Examples include the Toll-like receptor (TLR) and Nod-like receptor (NLR) families, which amplify inflammatory responses mediated by microbial components in host defense.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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