Analysis of TUB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2172-LGZ)

Introduction

Official Full Name: TUB bipartite transcription factor
Also known as: rd5; RDOB
This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Negishi Virus by Real-Time PCR Method (CAT#: STEM-MB-3614-LGZ)
Analysis of LCE1D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1120-LGZ)
Analysis of C2CD4D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0820-LGZ)
Analysis of UICLM Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1661-LGZ)
Analysis of CFAP126 Gene (Mutation) in Pheochromocytoma by RT-qPCR (CAT#: STEM-MT-0158-LGZ)
Analysis of Edwardsiella Ictaluri by Real-Time PCR Method (CAT#: STEM-MB-3075-LGZ)
Analysis of OPTC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0383-LGZ)
Analysis of MIR34A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1339-LGZ)