TAL can decompose tyrosine to produce 4-coumaric acid, and the absorbance of the reaction solution at 333 nm increases as the concentration of 4-coumaric acid increases. The TAL activity can be calculated according to the rate of absorbance change.
Applications
It is suitable for the detection of tyrosine ammonia lyase activity in fruit juice, plant and animal tissue samples.
Procedure
1. Prepare standard samples and experimental samples. 2. Add reaction reagents in order for reaction. 3. Measure the absorbance of each tube. 4. Make the mark curve and calculate the result.
Materials
• Sample Type: fruit juice, plant and animal tissue samples