Analysis of Mitochondrial Complex Ⅴ(F0F1-ATPase/ATP Synthase) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1479-LGZ)

Introduction

Sensitivity: 12.13 U/L
Detection range: 12.13-74.21 U/L
Precision: inter-lot difference of 6.5%, intra-lot difference of 5.6%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 330-350 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

ATP was hydrolyzed by F0F1-ATP synthetase to produce ADP, which converted NADH into oxidized coenzyme Ⅰ(NAD) after enzyme conversion reaction. F0F1-ATPase activity was calculated by measuring the change rate of absorbance at 340 nm.

Applications

It is suitable to detect the enzyme activity of mitochondrial respiratory chain complex Ⅴ in animal tissues and cells.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissues and cells

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