Analysis of Catalase (CAT) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1501-LGZ)

Introduction

Sensitivity: 0.01 U/L
Detection range: 0.01 -6.51 U/L
Precision: inter-lot difference of 6.8%, intra-lot difference of 3.4%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials

Principle

In the detection system, catalase decomposes H2O2 to produce water and oxygen. The undecomposed hydrogen peroxide reacts in the presence of enzyme and fluorescent substance, and its fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.

Applications

It is suitable for detecting catalase activity in serum (plasma), animal and plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal and plant tissue samples

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