Analysis of Uric Acid (UA) by Fluorescence Microplate Reader (CAT#: STEM-MB-1502-LGZ)

Introduction

Sensitivity: 0.03 μmol/L
Detection range: 0.03 - 15 μmol/L
Precision: inter-lot difference of 7.2%, intra-lot difference of 1.5%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials

Principle

Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into a fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.

Applications

It is used to detect the content of uric acid in serum (plasma), urine and other liquid samples and animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), urine and other liquid samples and animal tissue samples

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