Analysis of Total Iron by Enzyme-labeled Instrument (CAT#: STEM-MB-1384-LGZ)

Introduction

Sensitivity: 0.4 μmol/L
Detection range: 0.4 - 50 μmol/L
Precision: inter-lot difference of 1.5%, intra-lot difference of 1.3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 590 - 600 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Under the action of the reducing agent, the iron ions in the sample were reduced to ferrous ions, which combined with the probe. The resulting material had a strong absorption peak at 593 nm wavelength, and its optical density was linearly related to the concentration of iron ions within a certain range.

Applications

It is suitable for the determination of iron ion in serum, animal and plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, animal and plant tissue samples

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