Analysis of Succinate Dehydrogenase (SDH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1473-LGZ)

SDH catalyzes the dehydrogenation of succinic acid to fumaric acid, while the electron transport material transmits the electron to 2, 6-dichlorophenol indophenol (DCPIP), and then the reduced DCPIP is reduced into an oxidized DCPIP. The reduced product has a characteristic absorption peak at 600 nm. SDH enzyme activity was calculated by the change of absorbance decline at 600nm.

It was used to detect the activity of succinate dehydrogenase (SDH) in animal tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue and cell samples