Analysis of Calcium (Ca) by Enzyme-labeled Instrument (CAT#: STEM-MB-1386-LGZ)

Introduction

Sensitivity: 0.07 mmol/L
Detection range: 0.07-1.2 mmol/L
Precision: inter-lot difference of 8.5%, intra-lot difference of 4.7%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 600-620 nm

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Principle Applications Procedure Materials

Principle

Calcium ions in the sample were combined with methyl thymol blue (MTB) in alkaline solution to form blue complex. The depth of its color is proportional to the concentration of calcium ions. The substance has a maximum absorption peak at 610 nm. The calcium content in the sample can be calculated by colorimetric comparison with the calcium standard of the same treatment.

Applications

Applicable to the detection of calcium content in serum (plasma), urine, tissue and other samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, urine, tissue and other samples

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