Glucose oxidase (GOD, EC 1.1.3.4) can catalyze the oxidation of glucose into gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to oxidize pigment sources to produce colored substances.
Applications
It is suitable for the determination of glucose content in whole blood, serum and plasma.
Procedure
1. Prepare standard samples and experimental samples. 2. Add reaction reagents in order for reaction. 3. Measure the absorbance of each tube. 4. Make the mark curve and calculate the result.