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Analysis of Glucose (Glu) by Enzyme-labeled Instrument (CAT#: STEM-MB-1376-LGZ)

Introduction

Sensitivity: 0.04 mmol/L
Detection range: 0.04-30 mmol/L
Precision: inter-lot difference of 2.3%, intra-lot difference of 1.9%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 500-510 nm

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Principle Applications Procedure Materials

Principle

Glucose oxidase (GOD, EC 1.1.3.4) can catalyze the oxidation of glucose into gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to oxidize pigment sources to produce colored substances.

Applications

It is suitable for the determination of glucose content in whole blood, serum and plasma.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: whole blood, serum and plasma

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