Analysis of Nitrite by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1341-LGZ)

Introduction

Sensitivity: 1.36 μmol/L
Detection range: 1.36-500 μmol/L
Precision: inter-lot difference of 3.3%, intra-lot difference of 2.6%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 550 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

The concentration of the azo compound produced by NO2- and the chromogenic agent has a linear relationship with the concentration of NO2-, and the concentration of NO2- can be known by colorimetric comparison.

Applications

For the determination of NO2- in serum, plasma, animal and plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, animal and plant tissue samples

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