Analysis of Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome c Reductase) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1478-LGZ)

Mitochondria, as organelles, are the "power factories" in cells and the main places for aerobic respiration of cells. Their function is to convert energy through oxidative phosphorylation and provide energy for cell activities. The oxidation process is carried out by four respiratory chain protein complexes (complex Ⅰ, Ⅱ, Ⅲ and Ⅳ) in the inner mitochondrial membrane. Mitochondrial respiratory chain complex Ⅲ is also known as cytochrome c reductase complex. Its main function is to oxidize reduced coenzyme Q10 formed by mitochondrial respiratory chain complex Ⅰ and Ⅱ to oxidizing coenzyme Q10. Mitochondrial respiratory chain complex Ⅲ converted oxidizing cytochrome c into reduced cytochrome c, resulting in increased absorbance at 550 nm. The enzyme activity was calculated by detecting the rate of increase in absorbance at 550 nm.

The enzyme activity of mitochondrial respiratory chain complex Ⅲ(coenzyme Q- cytochrome c reductase) in tissue samples was determined.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: tissue samples