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Analysis of UBXN11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0092-LGZ)

Introduction

Also known as: SOC; SOCI; COA-1; UBXD5; PP2243
This gene encodes a protein with a differentiated C-terminal UBX domain. The homologous protein in rat interacts with members of the Rnd subfamily of Rho GTPases at the cell periphery through its C-terminal region. It also interacts with several heterotrimeric G proteins via the G-α subunit, promoting Rho GTPase activation. It is thought to play a bidirectional role in promoting and inhibiting Rho activity through upstream signaling pathways. The 3' coding sequence of the gene contains a polymorphic region of 24nt tandem repeat sequence. Some transcripts contain 1.5 to 5 repeat units. Multiple transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Lung cancer, Small cell lung carcinoma, Hepatocellular carcinoma

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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