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Analysis of VEGFR-3/Flt-4 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0627-LGZ)

Introduction

Vascular endothelial growth factor receptor 3 (VEGFR-3), also known as Flt-4, is a tyrosine kinase receptor for VEGF-C and VEGF-D. This protein is involved in the formation and maintenance of lymphatic vessels in the lymphatic endothelium. High expression of VEGFR-3 can be found in lymphatic endothelial cells isolated from cancer tissues of lymphoma patients.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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