Analysis of ZNF215 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2203-LGZ)

Introduction

Official Full Name: zinc finger protein 215
Also known as: BAZ2; BAZ-2; ZSCAN43; ZKSCAN11
This gene is imprinted in a tissue-specific manner with preferential expression in the testis, and encodes a zinc finger protein that belongs to a family of zinc finger transcription factors. The encoded protein contains an N-terminal SRE-ZBP, Ctfin51, AW-1, and Number 18 (SCAN) domain, a kruppel-associated box A (KRABA) domain, and four C-terminal zinc finger domains. This gene is located within one of three regions on chromosome 11p15 associated with Beckwith-Wiedemann syndrome, called Beckwith-Wiedemann syndrome chromosome region-2 (BWSCR2), and is thought to play a role in the etiology of this disease.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of C11orf16 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2185-LGZ)
Analysis of RAD54L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0106-LGZ)
Analysis of MPC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0458-LGZ)
Analysis of OR52K2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2366-LGZ)
Analysis of Ehrlichia Ruminantium by Real-Time PCR Method (CAT#: STEM-MB-3350-LGZ)
Analysis of SP100 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1540-LGZ)
Analysis of Equine Influenza Virus (EIV) by Real-Time PCR Method (CAT#: STEM-MB-3174-LGZ)
Analysis of TRIM33 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1159-LGZ)