CFI75 LWD 16xW is a water immersion objective with low magnification, high NA and long working distance. When combined with Nikon's Eclipse FN1 microscope and a dedicated magnification module, this lens provides magnifications of 5.6x, 32x, and 64x. Because it allows observation from a low magnification wide field to a high magnification, high resolution field with a single objective, the lens is very suitable for patch clamp experiments. The lens has excellent infrared transmittance, and is also suitable for IR-DIC observation, and the high NA value of the objective lens combined with the confocal laser microscope can provide excellent image quality. The CFI75 Apochromat LWD 25x MP provides a high numerical aperture of 1.10 while still maintaining a long working distance of 2.0mm. It can correct the chromatic aberration in the near infrared range, and has a ring that can correct the chromatic aberration according to the depth of the sample. Combined with its 33° joystick pipette entry angle, it is ideal for deep imaging of living samples using multiphoton excitation and physiology research applications.