Characterization of cellular microvesicles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0735-WXH)

Introduction

Cellular microvesicles (100 nm-1µm) are shed into the circulation from many different cell types including; platelets, erythrocytes and endothelial cells. They have major potential as novel biomarkers as they have been shown to be implicated in a multitude of pathological conditions.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

Applications

NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.

Procedure

1. Sample preparation
2. Nanoparticle Tracking Analysis
3. NTA Data Processing, Display, and Interpretation

Materials

• Nanoparticle Tracking Analysis (NTA) instrument
• NanoSight instrument
• Nanoparticle Analyzer

Other recommended products

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0718-WXH)
Analysis of Adeno-associated virus (AAV) by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0749-WXH)
Investigation of Nanodiamonds intracellular transport by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0745-WXH)
Investigation of Extracellular transport of NPs (nanoparticles) by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0748-WXH)
Investigation of Polystyrene (PS) intracellular transport by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0742-WXH)
Study of Nanotoxicology by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0746-WXH)
Analysis of Extracellular Vesicles by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0714-WXH)
Characterization of Amyloid-β Self-Assembly by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0716-WXH)