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Circular dichroism (CD) to study protein structure (CAT#: STEM-MB-0242-WXH)

Introduction

Circular Dichroism (CD), also known as circular dichroism spectroscopy, is an analytical technique developed based on the principle that photoactive substances absorb different plane polarized light to study the spatial conformation of substances. Use plane polarized light to irradiate the photoactive substance, take the wavelength λ of the plane polarized light as the abscissa, and the difference between the absorbance of the photoactive substance to the left and right polarized light as the ordinate, and draw the wavelength-absorption coefficient difference curve to obtain the substance circular dichroism spectrum.




Applications

• Study protein secondary and higher order structure
• Study the effects of manufacturing, formulation, and storage conditions on protein conformation and stability

Procedure

1.Sample preparation
2.Circular dichroism detection
3.Data analysis
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