Degenerate PCR (CAT#: STEM-MB-0224-WXH)

Introduction

Degenerate PCR is a PCR method that uses degenerate primers to amplify unknown DNA sequences that are related to a known DNA sequence or to amplify a mixture of related sequences in one PCR reaction. Degenerate primers are just a mix of primers with similar sequences.

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Applications Procedure

Applications

Degenerate PCR is useful for identify new members of a gene family or orthologous genes from different organisms.

Procedure

General procedure:
1. Denaturation
2. Annealing
3. Extension

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