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Determination of Melting Point of Triterpenoids from Schleichera Oleosa by Capillary Method (CAT#: STEM-PPA-0118-YJL)

Introduction

Darjeeling foothills have many plants that are used as folklore medicines by the local people. Schleichera oleosa (Lou.) Oken, commonly known as Ceylon Oak belongs to the family of sapindaceae occurs naturally at the foot hills of Darjeeling and is used in traditional medicine for several indications from the time immemorial. It is generally used as analgesic, antibiotic and against dysentery. S. oleosa is a well known medicinal plant in the Teak forest of East Java and is also used in West Bengal in India, as a commercial host plant for obtaining sticklac for production of shellac. In parts of southern India, S. oleosa is a prominent bee plant for nectar and is used for production of animal feedstock. The oil obtained from its seed, called Kusum oil or Macassar oil is traditionally used for the cure of itch, acne, burns, other skin troubles, rheumatism (external massage), hair dressing and for promoting hair growth. Two triterpenoids, taraxerone and tricadenic acid A were isolated from the methanol extract of the outer bark of Schleichera oleosa available in Darjeeling foothills.




Principle

Melting point is a characteristic property of solid crystalline substance. It is the temperature at which the solid phase changes to the liquid phase. This phenomenon occurs when the substance is heated.
In all major pharmacopoeias the open capillary method is described to determine the melting point (slip point) for fats, fatty acids, paraffin, and waxes. In a glass capillary tube open at both ends (1), 10 mm of sample is introduced (2), chilled to a given temperature and immersed into a water bath (3/4). The melting point with open capillary, also known as slip point or slip melting point, is the temperature at which the substance begins to rise in the capillary due to the effect of the increasing temperature and buoyancy.

Applications

Chemical industry; Cosmetic/pharmaceutical industry

Procedure

1. Prepare the substance.
2. Affix one of the capillaries to a thermometer.
3. Place the thermometer with an affixed capillary into a glass.
4. Increase the temperature.

Materials

• Sample Type: crystalline compounds
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