DIA Quantitative Proteomics to detect low-abundance protein (CAT#: STEM-MB-0089-WXH)

Introduction

Data independent acquisition (DIA) is one of the mass spectrometry acquisition technologies that have attracted attention in recent years and is a new development in quantitative proteomics. Compared with DDA, the advantage lies in the efficient determination of protein molecules with extremely low abundance in complex samples, which greatly improves the reliability of quantitative analysis. The principle is that during mass spectrometry data acquisition, all precursor ions and their daughter ions in each acquisition window are fragmented at high speed and cyclically before full scanning, instead of secondary fragment re-scanning after screening based on the signal intensity of precursor ions. Therefore, DIA has the advantages of high throughput, high resolution, high reproducibility, and accurate quantification, and the sample does not need to be graded on the machine, which greatly shortens the detection time of each sample, and is suitable for proteome research with large sample volume.