SILAC for quantitative proteomics (CAT#: STEM-MB-0085-WXH)

Introduction

Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. It is a popular method for quantitative proteomics.
The principle of SILAC is based on metabolically incorporating stable isotope labeled amino acids into the entire proteome.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Study of cell signaling, post translation modifications, protein–protein interaction and regulation of gene expression.
• Globle study of secreted proteins and secretory pathways.

Procedure

1. Cell culture markers (cultured in light and heavy amino acid medium respectively)
2. Protein purification
3. Mixed protein samples
4. Protein reduction and digestion
5. Detection by liquid chromatography-mass spectrometry (LC-MS)
6. Bioinformatics analysis

Notes

The customer provides or informs the cell line used in the experiment.

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