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Acetylation Quantitative Proteomics (CAT#: STEM-MB-0094-WXH)

Introduction

Protein acetylation (Acetylation) is a process in which a protein is catalyzed by an acetyltransferase (or non-enzyme), and the acetyl group is transferred and added to a protein lysine residue or a protein N-terminal. important role in protein structure and gene expression. Acetylation modification is mainly divided into two categories: acetylation modification at the N-terminal of protein and acetylation modification on protein lysine. Most of the N-terminal acetylation modification occurs on eukaryotic proteins, catalyzed by N acetyltransferases (NATs); acetylation modification on lysine is a reversible process, mainly catalyzed by lysine acetylases (KATs ) and lysine deacetylases (KDACs).




Applications

• Research on acetylation modification and cellular gene expression regulation, epigenetic regulation, cell apoptosis, cell metabolism, signal transduction, protein stability and other physiological processes
• Research on the mechanism of metabolic diseases, metabolic disorders, and tumorigenesis caused by acetylation

Procedure

1. Sampling
2. Protein extraction
3. Digestion
4. Mark
5. Immunoenrichment of acetylated peptides using high-efficiency specific acetylated antibodies
6. Mass spectrometry detection
7. Data analysis

Notes

Customers provide test samples.

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