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Ubiquitination Quantitative Proteomics (CAT#: STEM-MB-0095-WXH)

Introduction

Ubiquitination is a process in which proteins are covalently modified by ubiquitin (polypeptide consisting of 76 amino acids) under the action of a series of special enzymes. The ubiquitin-proteasome system mediates eukaryotic degradation of 80% to 90% of proteins plays a key role in almost all eukaryotic cell activities and is an important post-translational modification




Applications

Research on various physiological processes involved in ubiquitin-proteasome degradation pathway

Procedure

1. Protein extraction
2. Proteolysis
3. Enrichment of ubiquitinated peptides
4. Mass spectrometry detection
5. Qualitative and quantitative database search
6. Data Analysis

Notes

Customers provide test samples.

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