iTRAQ Technology for quantitative proteomics (CAT#: STEM-MB-0084-WXH)

Introduction

iTRAQ (isobaric tag for relative and absolute quantitation), is an isobaric labeling method to determine the amount of proteins from different sources in just one single experiment by mass. This method is based on the covalent labeling of the N-terminus and side chain amines of peptides from protein digestions with tags of varying mass.This powerful new proteomics technology can reduce the potential variation in numerous mass spectrometry runs and thus enhance the precision of protein identification and quantification.
The advantages of this technology include wide applicability, high throughput, accurate results, high sensitivity, high efficiency and high degree of automation

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Principle Applications Procedure Materials Notes Related Products

Applications

• Protein identification and quantification
• Study drug targets and mechanisms
• Comparison of proteins in multiple different samples
• Signal transduction research
• Molecular marker discovery
• Protein interaction research
• Molecular mechanisms of disease phenotypes
• Drug target research
• Protein post-translational modification

Procedure

1. Hydrolyze protein samples to obtain peptides
2. Labeling Peptides Using iTRAQ Reagent
3. Tandem mass spectrometry analysis of protein samples
4. Data processing

Notes

Customers provide samples that meet the requirements.

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