Genotyping by Sequencing (GBS) (CAT#: STEM-MB-0056-WXH)

Introduction

Genotyping by Sequencing (GBS), which belongs to one of Reduced-Representation Genome Sequencing techniques, is a method to discover single nucleotide polymorphisms (SNP) in order to perform genotyping studies. GBS greatly reduces genome complexity by using restriction enzymes to cleave the DNA coupled with DNA-barcoded adapters. By choosing appropriate restriction enzymes, GBS can provide high SNP coverage in gene-rich regions of the genome in a highly cost-effective manner, and increase the number of tags in the assay. The sequenced portion of the genome is highly consistent within a population because restriction sites are generally conserved across species. This makes the GBS protocol highly suited for experiments that require surveying.