RACE technology for full-length gene acquisition (CAT#: STEM-MB-0067-WXH)

Introduction

Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell.
3'RACE principle: Use reverse transcription primers with special adapters to reverse transcribe total RNA, and design primers based on the adapter sequences on the reverse transcription primers and the known sequence of mRNA to amplify the 3' end sequence.
5'RACE principle: After removing the cap structure at the 5' end, the phosphate group is exposed at the 5' end of the mRNA. T4 RNA ligase was used to ligate the specific linker at the 5' end of the mRNA. After reverse transcription, design primers based on the linker sequence and the known sequence of the mRNA to amplify the reverse transcription product, and the sequence at the 5' end of the mRNA can be amplified.