Next-generation Gequencing (NGS) (CAT#: STEM-MB-0059-WXH)

Introduction

Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has revolutionized the biological sciences, allowing labs to perform a wide variety of applications and study biological systems at a level never before possible.
Today's complex genomics questions demand a depth of information beyond the capacity of traditional DNA sequencing technologies. NGS has filled that gap and become an everyday tool to address these questions.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Rapidly sequence whole genomes
• Deeply sequence target regions
• Utilize RNA sequencing (RNA-Seq) to discover novel RNA variants and splice sites, or quantify mRNAs for gene expression analysis
• Analyze epigenetic factors such as genome-wide DNA methylation and DNA-protein interactions
• Sequence cancer samples to study rare somatic variants, tumor subclones, and more
• Study the human microbiome
• Identify novel pathogens

Procedure

1.DNA Isolation
2.Library Prep
3.Sequencing
4.Data Analysis

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