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Whole-genome Sequencing (WGS) (CAT#: STEM-MB-0048-WXH)

Introduction

Whole-genome resequencing (WGS) is the process of sequencing the genome of different individuals of a species with existing Reference Sequence and using it as a basis for analysis of genetic variability at the individual or population level. By whole-genome resequencing, researchers can find a large number of single nucleotide polymorphism (SNP), copy number variation (CNV), insertion/deletion (InDel), structure variation (SV) and other variant loci. Based on these variant loci as molecular genetic markers, they are of great significance in guiding research on complex human diseases, economic traits and breeding of plants and animals, as well as the origin, domestication and population history dynamics of species. Whole-genome resequencing can comprehensively scan the variant information on the genome and mine a large number of biomarkers at one time. This technology is highly accurate, reproducible and precisely targeted, and has been widely used in genomic research of diseases and cancers.




Applications

• Investigation of the relationship between sequence variation and normal or disease phenotypes
• Research on human diseases and animal breeding genes

Procedure

1.Extraction of genomic DNA
2.DNA quality control
3.Library construction
4.High throughput sequencing
5.Sequencing data pre-processing
6.Reference sequence alignment

Notes

Customer provids tissue or DNA samples

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