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Whole-genome resequencing (WGS) is the process of sequencing the genome of different individuals of a species with existing Reference Sequence and using it as a basis for analysis of genetic variability at the individual or population level. By whole-genome resequencing, researchers can find a large number of single nucleotide polymorphism (SNP), copy number variation (CNV), insertion/deletion (InDel), structure variation (SV) and other variant loci. Based on these variant loci as molecular genetic markers, they are of great significance in guiding research on complex human diseases, economic traits and breeding of plants and animals, as well as the origin, domestication and population history dynamics of species. Whole-genome resequencing can comprehensively scan the variant information on the genome and mine a large number of biomarkers at one time. This technology is highly accurate, reproducible and precisely targeted, and has been widely used in genomic research of diseases and cancers.