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Reduced-representation sequencing (RRS), is an approach to generate genome-wide high-throughput sequencing data and obtain a large number of genetic polymorphism tag sequences to fully represent the whole genome information of the species. RRS not only simplifies the sequencing method, given only the digested fragments are sequenced, it also simplifies the sequenced genome. Therefore, it is widely used in molecular marker development, population genetic analysis, genetic map construction, QTL mapping, genome-wide association analysis and other population research and molecular breeding fields.