CUT&Tag Technology to study protein-DNA interaction (CAT#: STEM-MB-0062-WXH)

Introduction

CUT&Tag (Cleavage Under Targets and Tagmentation) is a new method for the study of protein-DNA interaction. It is a new generation of ultra-micro ChIP-Seq technology, which is suitable for protein research without ChIP-level antibodies. Compared with the traditional ChIP-Seq research method, this technology does not require operations such as cross-linking, ultrasonic fragmentation, end smoothing, and adapter connection, so it is time-saving and efficient, requires less sample volume, low background signal and good reproducibility and other advantages, and can even be used for single-cell level sequencing.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Study of protein-DNA interaction in the field of epigenetics;
• Study the binding or distribution sites of transcription factors on the whole genome;
• Study the distribution sites of histone modifications on the whole genome;
• Find the target genes regulated downstream of transcription factors.

Procedure

1. Binding cells to magnetic beads
2. Co-incubation of target protein and antibody
3. Add ChiTag (fusion protein of Protein A protein and Tn5 transposase)
4. Fragmentation
5. DNA extraction
6. PCR amplification
7. High-throughput sequencing

Notes

Customers provide tissue, cell and other samples and related information.

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