Target Sequencing (CAT#: STEM-MB-0073-WXH)

Introduction

Target sequencing is a technical means to use probe hybridization to enrich the DNA sequence of the exon region, and through high-throughput sequencing to discover mutations related to protein function variation. Compared with whole genome and exome sequencing, target sequencing is more economical and efficient. Target sequencing is mainly used for the study of large sample size and small regional variation, which is more conducive to the detection of low-frequency mutations, and is widely used in clinical inspection and drug use.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Focusing on specific regions of interest
• Targeted analysis of microbial genes
• Detecting rare variants, sequence-focused content from multiple samples in parallel using multiplexing
• Achieving comprehensive coverage, yet generating a smaller, more reasonable amount of data
• Identifying causative variants like SNPs and InDels across multiple genomic regions
• Fast turnaround time for more samples

Procedure

1.DNA Extraction
2.Library construction
3.In-solution hybridization capture
4.Sequencing
5.Data analysis

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