Chromatin Immunoprecipitation Sequecing (ChIP-seq) (CAT#: STEM-MB-0043-WXH)

Introduction

Chromatin immunoprecipitation sequecing (ChIP-seq), is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest.
The main advantages of ChIP-seq are the excellent base pair resolution and the reduced number of samples required.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Determining what kind of histone modification occurs at a specific position in the DNA strand
• Detection of the precise location of binding sites of RNA polymerase II and other trans-factors on the genome
• Investigating the relationship between histone covalent modification and gene expression
• CTCF Transcription Factor Research
• Examination of the interactions between proteins and nucleic acids

Procedure

1. DNA enrichment quality inspection
2. Library construction and sequencing
3. Data filtering quality control
4. Reference genome comparison
5. Data Analysis

Notes

Customers provide experimental samples.

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