Imaging Higher-order Chromatin Structures in Single Cells by Stochastic Optical Reconstruction Microscopy (CAT#: STEM-MIT-0387-LJX)

Introduction

Chromatin is the material in the nucleus that can be stained with basic dyes during the intercellular phase. The basic chemical component of chromatin is deoxyribonucleoprotein, which is a complex of DNA, histone, non-histone, and a small amount of RNA. It is the form in which the genetic material of interphase cells exists.
Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order chromatin structure at their epigenomic states is of great importance for understanding chromatin compaction and its subsequent effect on gene expression and various cellular processes. With the recent advances in super-resolution microscopy, the higher-order chromatin structure can now be directly visualized in situ down to the scale of ~30 nm.