Histological analysis of human glomerular disease by direct stochastic optical reconstruction microscopy (dSTORM) (CAT#: STEM-MIT-0378-LJX)

Introduction

The glomerulus is the blood filter, and the capillary walls of the glomerulus form the filter membrane. When circulating blood passes through the glomerular capillaries, water and small molecular solutes in the plasma, including a small amount of plasma proteins with small molecular weights, can filter into the cystic cavity of the renal sacs to form filtrate. Micropuncture experiments have proved that the filtrate in the glomerular is ultrafiltrate in the plasma.

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of stochastic optical reconstruction microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Imaging in two or three dimensions, in multiple colors, and even in living cells
Applied in many areas of the life sciences, and provides very high resolution images for many different needs from neuroscience to subcellular science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Human glomeruli

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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