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Life Technologies Attune NXT AFC2 w/ Attune NxT Autosampler. Blue And Red Lasers. Flow Cytometer, Thermo Fisher Scientific (CAT#: STEM-CA-0399-LGZ)

Highlights

1. The innovative combination of acoustic focusing technology and traditional hydrodynamic focusing technology makes the acquisition speed 10 times faster than other flow cytometers. Reduced time required to get results.
2. Real 35,000 events/sec (+10% coincidence rate).
3. The maximum electron speed is 65,000 times/second.

Cat Number: STEM-CA-0399-LGZ

Application: The tune NxT flow cytometer is ideal for immunophenotyping and signaling studies, cell cycle analysis, rare event detection, stem cell analysis, cancer and apoptosis studies, microbial analysis, and more.

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Description

The manufacturer of Life Technologies Attune NXT AFC2 w/ Attune NxT Autosampler. Blue And Red Lasers. Flow Cytometer is Thermo Fisher Scientific. Model is Attune NXT AFC2. The 2017-2018 tune NxT flow cytometer has expanded capabilities to move your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the tune NxT flow cytometer provide adaptable optical configuration options so you can get the most from your multicolor analysis.

Specification

Condition: used
Lasers:
Violet: 405 nM (50 mW)
Blue: 488 nM (50 mW)
Yellow: 561 nM (50 mW)
Red: 637 nM (100 mW)
Filters:
VL1: 440/50
VL2: 512/25
VL3: 603/48
VL4: 710/50RL1: 670/14
RL2: 720/30
RL3: 780/60YL1: 585/16
YL2: 620/15
YL3: 695/40
YL4: 780/60BL1: 530/30
BL2: 590/40
BL3: 695/40
Scatter:
Forward scatter: Photodiode detector with 488/10
Side scatter: PMT with 488/10
Control and Analysis:
Dell Desktop with Intel CORE i7 with Windows 7
Attune NxT Software version 2.2
Dell Flat Panel LCD monitor
Automated maintenance:
startup (less than 15 min), shutdown, rinse, wash, debubble, deep clean, monthly decontamination
Gates: standard and customizable gates
Graphics resolution: publication-quality images (customizable)
Probe Design: Intelligent probe design minimizes clogging and carryover (less than 1%) – helps prevent damage to the instrument
Sample Mixing: Mixes sample by aspiration to maintain sample homogeneity and cell viability
Laser: Simmer mode: automatic shutoff prolongs laser usage lifetime by 10xPre-aligned and welded fiber to laser interfacePre-aligned fiber to beam-shaping optics (BSO) interface (alignment-free)All lasers spatially separatedFocus lens per laser: power where you want itFlat-top laser specified at the flow cell
Benefits of flat-top lasers
• ~50% reduction in scattered light
• No problems with lateral shift in alignment

Features

1. The innovative combination of acoustic focusing technology and traditional hydrodynamic focusing technology makes the acquisition speed 10 times faster than other flow cytometers. Reduced time required to get results.
2. Real 35,000 events/sec (+10% coincidence rate).
3. The maximum electron speed is 65,000 times/second.
4. Analyze small or limited samples faster than ever before.
5. Efficient traditional long-term analysis.
6. The modular design of the instrument provides multiple upgrade options for flexibility to meet future capacity needs.
7. The instrument has a small footprint, requires minimal bench space, and can even be used in a cell culture hood.
8. Can easily run most samples including cardiomyocytes. The system is designed with an anti-clogging syringe drive system that pushes samples at pressures up to 75 psi.
9. Flexibility in sample concentration and sample type.
10. Eliminates time-consuming concentration steps, no cleaning, and no lysozyme filtration kits.
11. Analysis of diluted samples including cerebrospinal fluid, stem cells, mouse bone marrow, mouse blood and fine needle aspirate.
12. Obtain a large number of samples in the shortest time.

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